Parametric optimalization of submerged fermantation conditions for xylanase production Bacillus cereus BSA1 through Taguchi Methodology

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Asish Mandal
Sanjay Kar
Tapan Dutta
Bikas Ranjan Pati
Keshab Chandra Mondal
Pradeep Kumar Das Mohapatra

Abstract

Extracellular xylanase production of Bacillus cereus BSA1 was optimized under submerged fermentation using Taguchi orthogonal array (OA). An L18 layout of OA was constructed at three-levels of six factors, i.e. temperature, pH, and xylan, Na2HPO4, NH4NO3 and NaCl concentrations, influencing the xylanase synthesis. The enzyme production was studied in 18 parallel batch systems using different levels of each factor. The results were processed with Qualitek-4 software using ‘bigger is better’ quality character, and combination of 35 oC; pH 6.0; and xylan 0.5; NH4NO3 0.5, Na2HPO4, 0.1; NaCl 0.05 concentrations (in w/v %) with a predictive xylanase production of 7.404 U/ml was obtained. Fermentation experiment was performed for further validating the statistical output, and it resulted 10.24% in the xylanase yield (from 6.44 U/ml to 7.10 U/ml) as compared to one variable at a time (OVAT) design. Interaction effects of the factors individually and in combination can be evaluated by using Taguchi method design of experiment.

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How to Cite
Mandal, A., Kar, S., Dutta, T., Pati, B. R., Mondal, K. C. and Mohapatra, P. K. D. (2015) “Parametric optimalization of submerged fermantation conditions for xylanase production Bacillus cereus BSA1 through Taguchi Methodology”, Acta Biologica Szegediensis, 59(2), pp. 189–195. Available at: https://abs.bibl.u-szeged.hu/index.php/abs/article/view/2881 (Accessed: 7 October 2022).
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