Expression and production optimization of the cationic antimicrobial peptide - indolicidin by the recombinant E. coli C41 (DE3) clones
DOI:
https://doi.org/10.14232/abs.2018.1.61-66Keywords:
cationic antimicrobial peptide, drug resistance, indolicidin, optimizationAbstract
The cytoplasmic granules of bovine neutrophils naturally possess indolicidin - a promising cationic antimicrobial peptide as it displays inherent inhibitory activities against a broad type of microbial pathogens. In this study, a shake flask level production and expression optimizations of the indolicidin by the recombinant Escherichia coli C41 (DE3) clones (transformed with pET21a(+) plasmid carrying indolicidin gene) were carried out under standard conditions, as to determine the conditions required for maximal production. It was determined that a concentration of 1 mM of IPTG was effective, the 2×YT with salts and LB media at pH 7.5 with 3-6 h of incubation were required for maximal indolicidin expression.
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