Nephroprotective efficacy of Asparagus racemosus root extract on acetaminophen-induced renal injury in rats

Main Article Content

Suchismita Roy
Shrabani Pradhan
Shreya Mandal
Koushik Das
Dilip Kumar Nandi


Acetaminophen-induced renal necrosis and insufficiency occurs in patients with acetaminophen overdose. Renal failure is rapidly assuming epidemic proportions globally. In absence of reliable and effective nephroprotective drugs, strategies towards exploring alternative therapies for treatment of kidney diseases are essential. Asparagus racemosus is a medicinal plant used for treatment of various ailments. This research was undertaken to investigate the protective effect of ethanol fraction of A. racemosus roots extract in acetaminophen-induced uraemia and renal failure in rats. Rats were co-administered with acetaminophen injection and oral administration of A. racemosus roots extract in an attempt of protection against renal failure. Uremic biomarkers significantly decreased, and elevated levels of antioxidant enzymes were found, in the animals treated with ethanol fraction of A. racemosus when compared with acetaminophen treated uremic animals. Also, histology of kidneys showed control like structure in animals treated with this extract but severe damage in the uremic animals. HPLC analysis of the ethanol fraction of A. racemosus roots extract revealed eight compounds out of which one had a retention time near to the quercetin standard. It may be concluded that this extract of A. racemosus has therapeutically useful nephroprotective potential.


Download data is not yet available.

Article Details

How to Cite
Roy, S., Pradhan, S., Mandal, S., Das, K. and Nandi, D. K. (2018) “Nephroprotective efficacy of Asparagus racemosus root extract on acetaminophen-induced renal injury in rats”, Acta Biologica Szegediensis, 62(1), pp. 17–23. doi: 10.14232/abs.2018.1.17-23.


Beers RF, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide of catalase. J Biol Chem 195:133-140.

Burtis CA, Ashwood ER (1999) Tietz Textbook of Clinical Chemistry. 3rd ed, W. B. Saunders Co., Philadelphia. pp. 809-861.

Das K, Chakraborty PP, Ghosh D, Nandi DK (2010a) Protective effect of aqueous extract of Terminalia arjuna on dehydrating induced oxidative stress and uremia in male rat. Iran J Pharm Res 9:153-161.

Das K, Tulsian T, Samanta P, Nandi DK (2010b) Effect of extract of Withania sominifera on dehydration induced oxidative stress related uremia of male rat. Saudi J Kidney Dis Transpl 21:75-80.

Elkarib AO (2014) Impact of dehydro epiandrosterone in prevention of paracetamol induced nephrotoxicity in rats. Am Med J 5:16-27.

Fored CM, Ejerblad E, Lindbland P, Fryzek JP, Dickman PW, Signorello LB, Lipworth L, Elinder CG, Blot WJ, McLaughlin JK, Zack MM, Nyren O (2001) Acetaminophen, aspirin, and chronic renal failure. New Eng J Med 345:1801-1808.

Goyal RK, Singh J, Lal H (2003) Asparagus racemosus – An update. Indian J Med Sci 57:408-414.

Hardman JG, Limbird LE, Gilman AG (2001) Goodman and Gilman’s The Pharmacological Basis of Therapeutics, 10th ed. Chapter 4. McGraw-Hill Medical Publishing Division, New York.

Linares V, Belles M, Albina ML, Sirvent JJ, Sanchez DJ, Domingo JL (2006). Assessment of the pro-oxidant activity of uranium in kidney and testis of rats. Toxicol Lett 167:152-161.

Mandal A, Mandal S, Roy S, Patra A, Pradhan S, Das K, Paul T, Mondal KC, Nandi DK (2013c) Assessment of efficacy of a potential probiotic strain and its antiuremic and antioxidative activities. e-SPEN J 8:155-163.

Mansour HH, Hafez HF, Fahmy NM (2006) Silymarin modulates cisplatin-induced oxidative stress and hepatotoxicity in rats. J Biochem Mol Biol 39:656-661.

Marklund S, Marklund G (1947) Involvement of superoxide anion radical in autotoxidation of pyrogallol and a convenient assay of superoxide dismutase. Eur J Biochem 47:469-474.

McLaughlin JK, Lipworth L, Chow WH, Blot WJ (1998) Analgesic use and chronic renal failure: a critical review of the epidemiologic literature. Kidney Int 54:679-686.

Mukherjee KL (2010) Medical Laboratory Technology. 2nd ed. Chapter 4. New Delhi, Tata McGraw-Hill Education.

NIH (1985) NIH Guide for the Care and Use of Laboratory Animals. NIH Publication No.85-23. Bethesda MD: US Department of Health, Education and Welfare, National Institute of Health.

O’Grady JG (1997) Paracetamol-induced liver failure: prevention and management. J Hepatol 26:41-46.

Ohkawa H, Ohishi N, Yagi K (1976) Assay for lipid peroxidation in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351-358.

Olfert ED, Cross BM, McWilliam AA (1993) Guide to Care and Use of Experimental Animals, 2nd ed. Chapter 2. Ottawa, CCAC.

Pradhan S, Mandal S, Roy S, Mandal A, Das K, Nandi DK (2013) Attenuation of uremia by orally feeding alphalipoic acid on acetaminophen induced uremic male rats. Saudi Pharm J 21:187-192.

Prescott L (2005) Oral or intravenous N-acetylcysteine for acetaminophen poisoning? Ann Emerg Med 45:409-413.

Roy S, Das K, Mandal S, Pradhan S, Patra A, Nandi DK (2013) Crude extract from root of Asparagus racemosus ameliorates acetaminophen induced uremic rats. Int J Pharm Sci Res 4: 3004-3012.

Roy S, Pradhan S, Das K, Mandal A, Mandal S, Patra A, Samanta A, Sinha B, Nandi DK (2015) Acetaminophen induced kidney failure in rats: a dose response study. J Biol Sci 15:187-193.

Roy S, Das K, Mandal S, Pradhan S, Patra A, Samanta A, Mandal A, Kar S, Nandi DK (2014a) Asparagus racemosus roots ameliorates acetaminophen induced hepatotoxicity in rats: an experimental, biochemical and histological study. Int J Recent Sci Res 5:1192-1197.

Roy S, Pradhan S, Mandal S, Das K, Patra A, Samanta A, Sinha B, Kar S, Nandi DK (2014b). Phytochemical analysis, antimicrobial activity and assessment of potential compounds by thin layer chromatography of ethanol fraction of Asparagus racemosus roots. Int J Pharm Pharm Sci 6:367-370.

da Silva Melo DA, Saciura VC, Poloni JA, Oliveira CS, Filho JC, Padilha RZ, Reichel CL, Neto EJ, Oliveira RM, D'avila LC, Kessler A, de Oliveira JR (2006) Evaluation of renal enzymuria and cellular excretion as a marker of acute nephrotoxicity due to an overdose of acetaminophen in Wistar rats. Clin Chim Acta 373:88-91.

Slitt AML, Dominick PK, Roberts JC, Cohen SD (2005) Effects of ribose cysteine pretreatment on hepatic and renal acetaminophen metabolite formation and glutathione depletion. Basic Clin Pharmacol Toxicol 96:487-494.

Somani SM, Husain K, Whitworth C, Trammell GL, Malafa M, Rybak LP (2000) Dose-dependent protection by lipoic acid against cisplatin-induced nephrotoxicity in rats: antioxidant defense system. Pharmacol Toxicol 86: 234-241.

Sunderman FW (1959) Studies on the serum proteins. IV. The dye-binding of purified serum proteins separated by continuous-flow electrophoresis. Clin Chem 5:171-185.

Visavadiya NP, Narsimhacharya AV (2005). Hypolipidemic and antioxidant activities of Asparagus racemosus in hypercholesteromic rats. Indian J Pharmacol 37:376-380.

Youdin KA, Deans SG, Finlayson HJ (2002) The antioxidant properties of thyme (Thymus zygis L.) essential oil: an inhibitor of lipid peroxidation and a free radical scavenger. J Essen Oil Res 14: 210-215.