Single nucleotide polymorphism C994g of the cytochrome P450 gene possess pleiotropic effects in Bos taurus, L.

Olena Fedota; Nina Puzik; Inessa Skrypkina; Volodymyr Babalyan; Larysa Mitiohlo; Serhii Ruban; Serhii Belyaev; Oleksandr O. Borshch; Oleksandr V. Borshch

doi:10.14232/abs.2022.1.7-15

Authors

Olena Fedota V. N. Karazin Kharkiv National University , Department of Obstetrics and Gynecology https://orcid.org/0000-0001-9659-383X
Nina Puzik V. N. Karazin Kharkiv National University , Department of Obstetrics and Gynecology, ,Kharkiv National Medical University https://orcid.org/0000-0002-3388-0038
Inessa Skrypkina Laboratory of Biosynthesis of Nucleic Acids, Institute of Molecular Biology and Genetics , National Academy of Sciences of Ukraine https://orcid.org/0000-0003-0529-6069
Volodymyr Babalyan Department of Orthopedics and Trauma, Kharkiv Medical Academy of Postgraduate Education
Larysa Mitiohlo V. N. Karazin Kharkiv National University , Department of Obstetrics and Gynecology, Research Farm “Nyva”, Institute of Animal Breeding and Genetics nd. a. M.V. Zubets of NAAN, Hristinivka, Hristinivs'kiy District, Ukraine https://orcid.org/0000-0001-5941-5700
Serhii Ruban National University of Life and Environmental Sciences of Ukraine , Department of Genetics, Breeding and Reproductive Biotechnology https://orcid.org/0000-0002-1366-9569
Serhii Belyaev Department of Orthopedics and Trauma, Kharkiv Medical Academy of Postgraduate Education
Oleksandr O. Borshch National University of Life and Environmental Sciences of Ukraine , Department of Genetics, Breeding and Reproductive Biotechnology, ,Bila Tserkva National Agrarian University , Departmet of Technology Milk and Meat Production
Oleksandr V. Borshch Bila Tserkva National Agrarian University , Departmet of Technology Milk and Meat Production

DOI:

https://doi.org/10.14232/abs.2022.1.7-15

Keywords:

cattle, cytochrome P450, mycotoxin contamination, pleiotropic eff ect, single-nucleotide polymorphisms

Abstract

The chronic consumption of mycotoxin-contaminated forage tends to the weight loss of young cattle, decreasing the reproductive success of adult animals, milk yield, and tolerance to high temperatures. The aim of this paper was to study the eff ects of the SNP C994G in the CYP3A28 gene on the productive and reproductive characteristics of dairy and beef breeds. Cows were measured by body weight dynamics, exterior, reproduction, milk production traits, bone mineral density, bulls were analyzed estimating their progeny traits. Dairy cows received dietary betaine supplement with the measurement of homocysteine levels. Molecular genetic was performed by PCR-RFLP method. The effects of SNP C994G were shown in relation to the udder size, the constitution and birth weight of Abredin-Angus cows, as well as the fat and protein content of Ukrainian Red-and-White Dairy cows milk. The higher sensitivity of the GG genotype to less homocysteine level after the addition of betaine was shown. Given the identifi ed correspondence, pleiotropic eff ects of CYP3A28 gene can be concluded. A microscopic analysis of forage supported the findings in relation to greater efficiency of the CC genotype under conditions of low mycotoxins contamination, to which CYP3A28 is selective.