Indirect shoot organogenesis and plantlets regeneration from stem of ornamental Dieffenbachia maculata cv. Marianna

Abstract

The present study reports a simple protocol for indirect shoot organogenesis and plantlets regeneration of Dieffenbachia maculata cv. Marianna using stem segments from in vitro shoot culture. Different concentrations and combinations of indole-3-acetic acid (IAA), 1- naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2, 4-D), 6-benzyladenine (BA), and kinetin (Kin) were used for callus induction and shoot organogenesis. The frequency of callus formation reached 87% and the highest number of shoots per callus was 4.8 for explants cultured on Murashige and Skoog (1962) medium supplemented with 15 mg I'1 BA + 15 mg I'1 NAA. Shoots were further grown and rooted on MS hormone free medium. Regenerated plantlets were successfully acclimatized in greenhouse with 100% survival rate.