Attempts to produce transgenic Beta vulgaris L. plants via combined gene transfer methods

Abstract

We have started to elaborate a general transformation and plant regeneration system for Beta vulgaris L. using combined gene transfer procedure. Three methods were tested: particle bombardment with pure wolfram micro-carriers prior to Agrobacterium treatment, vortexing and infiltration of explants in Agrobacterium suspension, halving of explants. The histochemical analysis of transient and stabile gus-gene expression showed the beneficial effect of explant halving and infiltration in Agrobacterium suspension.