Determination and uncertainty analysis of inorganic arsenic in rice by UHPLC-ICPMS

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Rajesh Rangasamy
Dheeraj Kumar Tyagi
Rahul Raveendran
Ananda Gupta

Abstract

The present study arose from the need of to determine inorganic arsenic (iAs) at low levels in rice. Ultra-high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (UHPLC-ICPMS) using Kinetic Energy Discrimination (KED) mode to eliminate spectral interferences was used for analysis of iAs. Sample preparation involves extraction of inorganic arsenic (sum of As3+ and As5+) with water by heating at 90 °C for 5 min in water bath. Separation is accomplished with a reversed-phase ion pack column using a gradient chromatographic method followed by ICPMS analysis within 5 min. The method was validated in accordance with Commission Regulation (EU) No 836/2011 and performance characteristics were verified. Acceptable values were obtained for specificity, repeatability (HorRatr < 0.6), within-lab reproducibility (HorRatR < 0.3) with recovery 80-90%, limit of quantification (0.02 mg/kg), fitness-for-purpose and trueness (using CRM); thus, the method can be considered for official control purposes.

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How to Cite
Rangasamy, R., Malekadi, P. K., Mohamed, D. P., Tyagi, D. K., Raveendran, R. and Gupta, A. (2021) “Determination and uncertainty analysis of inorganic arsenic in rice by UHPLC-ICPMS”, Acta Biologica Szegediensis, 64(2), pp. 251–259. doi: 10.14232/abs.2020.2.251-259.
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