Determination and uncertainty analysis of inorganic arsenic in rice by UHPLC-ICPMS

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Rajesh Rangasamy
Dheeraj Kumar Tyagi
Rahul Raveendran
Ananda Gupta


The present study arose from the need of to determine inorganic arsenic (iAs) at low levels in rice. Ultra-high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (UHPLC-ICPMS) using Kinetic Energy Discrimination (KED) mode to eliminate spectral interferences was used for analysis of iAs. Sample preparation involves extraction of inorganic arsenic (sum of As3+ and As5+) with water by heating at 90 °C for 5 min in water bath. Separation is accomplished with a reversed-phase ion pack column using a gradient chromatographic method followed by ICPMS analysis within 5 min. The method was validated in accordance with Commission Regulation (EU) No 836/2011 and performance characteristics were verified. Acceptable values were obtained for specificity, repeatability (HorRatr < 0.6), within-lab reproducibility (HorRatR < 0.3) with recovery 80-90%, limit of quantification (0.02 mg/kg), fitness-for-purpose and trueness (using CRM); thus, the method can be considered for official control purposes.


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Rangasamy, R., Malekadi, P. K., Mohamed, D. P., Tyagi, D. K., Raveendran, R. and Gupta, A. (2021) “Determination and uncertainty analysis of inorganic arsenic in rice by UHPLC-ICPMS”, Acta Biologica Szegediensis, 64(2), pp. 251–259. doi: 10.14232/abs.2020.2.251-259.