@article{Cruz_Larentis_Vismara_Vismara_Freitas_Mazaro_2022, title={Action of Ganoderma lucidum mycelial growth filtrates on Erysiphe diffusa and embryotoxicity assessment in a chicken embryo model}, volume={65}, url={https://abs.bibl.u-szeged.hu/index.php/abs/article/view/3193}, DOI={10.14232/abs.2021.1.47-57}, abstractNote={<p>This work aimed to evaluate the antimicrobial effect of <em>Ganoderma lucidum</em> mycelial growth filtrates (MGF) on the phytopathogen <em>Erysiphe diffusa</em> and their potential effects on the embryonic development of <em>Gallus gallus</em>. The antimicrobial activity was evaluated on <em>E. diffusa</em> spores by the microdilution broth method. To evaluate embryotoxic and teratogenic effects, fertile eggs of <em>G. gallus</em> received injections of solutions containing the filtrates of <em>G. lucidum</em> through the air chamber. After three days of incubation, we opened the eggs and evaluated egg viability, embryo survival, malformation occurrence, embryonic staging and heart rate. Live embryos were prepared using whole mount technique and the morphological analysis was performed. We used the generalized linear model to fit embryotoxicity and teratogenicity data. We verified that <em>G. lucidum</em> MGF showed inhibitory activity in vitro against <em>E. diffusa</em> and the minimum inhibitory concentrations ranged from 5 to 10 mg/mL. We could also observe that the filtrates did not present embryotoxic or teratogenic effects on the early embryonic development of <em>G. gallus</em>, but induced significant differences in the embryonic mean heart rate and on the stage of embryonic development.</p>}, number={1}, journal={Acta Biologica Szegediensis}, author={Cruz, Mycheli P. da and Larentis, Lucas T. and Vismara, Edgar de S. and Vismara, Lilian de S. and Freitas, Patricia F. de and Mazaro, Sérgio M.}, year={2022}, month={Mar.}, pages={47–57} }