TY - JOUR AU - Selvam, Kanesan Panneer AU - Nambirajan, Guruvu AU - Annamalai, Balasubramaniam AU - Alaidarous, Mohammed AU - Alshehri, Bader Mohammed AU - Bin Dukhyil, Abdul Aziz A. AU - Shobana, Coimbatore Subramanian AU - Manikandan, Palanisamy PY - 2018/08/23 Y2 - 2024/03/29 TI - Expression and production optimization of the cationic antimicrobial peptide - indolicidin by the recombinant E. coli C41 (DE3) clones JF - Acta Biologica Szegediensis JA - Acta Biol Szeged VL - 62 IS - 1 SE - Articles DO - 10.14232/abs.2018.1.61-66 UR - https://abs.bibl.u-szeged.hu/index.php/abs/article/view/2944 SP - 61-66 AB - <p>The cytoplasmic granules of bovine neutrophils naturally possess indolicidin - a promising cationic antimicrobial peptide as it displays inherent inhibitory activities against a broad type of microbial pathogens. In this study, a shake flask level production and expression optimizations of the indolicidin by the recombinant <em>Escherichia coli</em> C41 (DE3) clones (transformed with pET21a(+) plasmid carrying indolicidin gene) were carried out under standard conditions, as to determine the conditions required for maximal production. It was determined that a concentration of 1 mM of IPTG was effective, the 2×YT with salts and LB media at pH 7.5 with 3-6 h of incubation were required for maximal indolicidin expression.</p> ER -