Antioxidative defence mechanism contributes to desiccation tolerance in Haberlea rhodopensis population naturally exposed to high irradiation

Abstract

Drought induced stress is one of the most important among the environmental challenges. Haberlea rhodopensis, a chlorophyll-retaining resurrection plant, can survive desiccation to air-dry stage in its usual low irradiance habitat (“shade” plants). Nevertheless, in the past years, some populations living under high irradiance (“sun” plants) have been also discovered with the same ability to survive dehydration. In order to clarify the adaptation mechanisms to a high irradiation habitat, superoxide dismutase (SOD) activity determined by activity staining on polyacrylamide gels and malondialdehyde (MDA) content of sun and shade plants collected from high and low irradiance environment, respectively, were studied. Desiccation induced a significantly higher induction in SOD activity and thus a smaller increase in the MDA content in sun compared to shade plants. The MDA content and SOD activity was restored in both sun and shade plants after six-day rehydration. Nevertheless, the SOD activity remained higher in rehydrated sun leaves compared to the well-hydrated initial stage. The early enhancement of SOD activity in dehydrating sun plants contributes to the higher stress tolerance of these populations.